One-step generation of conditional and reversible gene knockouts

CRISPR technology has made genome editing widely accessible in model organisms and cells. However, conditional gene inactivation in diploid cells is still difficult to achieve. Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid, and scalable method for bi-allelic conditional...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Andersson-Rolf, Amanda, Mustata, Roxana C., Merenda, Alessandra, Kim, Jihoon, Perera, Sajith, Grego, Tiago, Andrews, Katie, Tremble, Katie, Silva, José C.R., Fink, Juergen, Skarnes, William C., Koo, Bon-Kyoung
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5777571/
https://ncbi.nlm.nih.gov/pubmed/28135257
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4156
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