One-step generation of conditional and reversible gene knockouts

CRISPR technology has made genome editing widely accessible in model organisms and cells. However, conditional gene inactivation in diploid cells is still difficult to achieve. Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid, and scalable method for bi-allelic conditional...

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Publicado en:Nat Methods
Autores principales: Andersson-Rolf, Amanda, Mustata, Roxana C., Merenda, Alessandra, Kim, Jihoon, Perera, Sajith, Grego, Tiago, Andrews, Katie, Tremble, Katie, Silva, José C.R., Fink, Juergen, Skarnes, William C., Koo, Bon-Kyoung
Formato: Artigo
Lenguaje:Inglês
Publicado: 2017
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5777571/
https://ncbi.nlm.nih.gov/pubmed/28135257
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4156
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