One-step generation of conditional and reversible gene knockouts

CRISPR technology has made genome editing widely accessible in model organisms and cells. However, conditional gene inactivation in diploid cells is still difficult to achieve. Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid, and scalable method for bi-allelic conditional...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nat Methods
Prif Awduron: Andersson-Rolf, Amanda, Mustata, Roxana C., Merenda, Alessandra, Kim, Jihoon, Perera, Sajith, Grego, Tiago, Andrews, Katie, Tremble, Katie, Silva, José C.R., Fink, Juergen, Skarnes, William C., Koo, Bon-Kyoung
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2017
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5777571/
https://ncbi.nlm.nih.gov/pubmed/28135257
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4156
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