Visualizing long-term single-molecule dynamics in vivo by stochastic protein labeling

Our ability to unambiguously image and track individual molecules in live cells is limited by packing of multiple copies of labeled molecules within the resolution limit. Here we devise a universal genetic strategy to precisely control copy number of fluorescently labeled molecules in a cell. This s...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Main Authors: Liu, Hui, Dong, Peng, Ioannou, Maria S., Li, Li, Shea, Jamien, Pasolli, H. Amalia, Grimm, Jonathan B., Rivlin, Patricia K., Lavis, Luke D., Koyama, Minoru, Liu, Zhe
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 2018
Assuntos:
Biological Sciences
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5777047/
https://ncbi.nlm.nih.gov/pubmed/29284749
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1713895115
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