Identification of a ubiquitin-binding interface using Rosetta and DEER

ExoU is a type III-secreted cytotoxin expressing A(2) phospholipase activity when injected into eukaryotic target cells by the bacterium Pseudomonas aeruginosa. The enzymatic activity of ExoU is undetectable in vitro unless ubiquitin, a required cofactor, is added to the reaction. The role of ubiqui...

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Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Tessmer, Maxx H., Anderson, David M., Pickrum, Adam M., Riegert, Molly O., Moretti, Rocco, Meiler, Jens, Feix, Jimmy B., Frank, Dara W.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2018
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5776994/
https://ncbi.nlm.nih.gov/pubmed/29295930
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1716861115
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