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Label-free Imaging and Bending Analysis of Microtubules by ROCS Microscopy and Optical Trapping

Mechanical manipulation of single cytoskeleton filaments and their monitoring over long times is difficult because of fluorescence bleaching or phototoxic protein degradation. The integration of label-free microscopy techniques, capable of imaging freely diffusing, weak scatterers such as microtubul...

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Détails bibliographiques
Publié dans:Biophys J
Auteurs principaux: Koch, Matthias D., Rohrbach, Alexander
Format: Artigo
Langue:Inglês
Publié: The Biophysical Society 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5773756/
https://ncbi.nlm.nih.gov/pubmed/29320684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2017.10.036
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