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Label-free Imaging and Bending Analysis of Microtubules by ROCS Microscopy and Optical Trapping

Mechanical manipulation of single cytoskeleton filaments and their monitoring over long times is difficult because of fluorescence bleaching or phototoxic protein degradation. The integration of label-free microscopy techniques, capable of imaging freely diffusing, weak scatterers such as microtubul...

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Detalles Bibliográficos
Publicado en:Biophys J
Main Authors: Koch, Matthias D., Rohrbach, Alexander
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2018
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5773756/
https://ncbi.nlm.nih.gov/pubmed/29320684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2017.10.036
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