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Application of a Schizosaccharomyces pombe Edc1-fused Dcp1–Dcp2 decapping enzyme for transcription start site mapping

Changes in the 5′ leader of an mRNA can have profound effects on its translational efficiency with little effect on abundance. Sequencing-based methods to accurately map the 5′ leader by identifying the first transcribed nucleotide rely on enzymatic removal of the 5′ eukaryotic cap structure by toba...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Paquette, David R., Mugridge, Jeffrey S., Weinberg, David E., Gross, John D.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5769751/
https://ncbi.nlm.nih.gov/pubmed/29101277
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.062737.117
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