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Application of a Schizosaccharomyces pombe Edc1-fused Dcp1–Dcp2 decapping enzyme for transcription start site mapping

Changes in the 5′ leader of an mRNA can have profound effects on its translational efficiency with little effect on abundance. Sequencing-based methods to accurately map the 5′ leader by identifying the first transcribed nucleotide rely on enzymatic removal of the 5′ eukaryotic cap structure by toba...

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Vydáno v:RNA
Hlavní autoři: Paquette, David R., Mugridge, Jeffrey S., Weinberg, David E., Gross, John D.
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5769751/
https://ncbi.nlm.nih.gov/pubmed/29101277
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.062737.117
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