Segmented cell analyses to measure redox states of autofluorescent NAD(P)H, FAD & Trp in cancer cells by FLIM

Multiphoton FLIM microscopy offers many opportunities to investigate processes in live cells, tissue and animal model systems. For redox measurements, FLIM data is mostly published by cell mean values and intensity-based redox ratios. Our method is based entirely on FLIM parameters generated by 3-de...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wallrabe, Horst, Svindrych, Zdenek, Alam, Shagufta R., Siller, Karsten H., Wang, Tianxiong, Kashatus, David, Hu, Song, Periasamy, Ammasi
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5758727/
https://ncbi.nlm.nih.gov/pubmed/29311591
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-18634-x
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