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The Organoid Reconstitution Assay (ORA) for the Functional Analysis of Intestinal Stem and Niche Cells

The intestinal epithelium is characterized by an extremely rapid turnover rate. In mammals, the entire epithelial lining is renewed within 4 - 5 days. Adult intestinal stem cells reside at the bottom of the crypts of Lieberkühn, are earmarked by expression of the Lgr5 gene, and preserve homeostasis...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Schewe, Matthias, Sacchetti, Andrea, Schmitt, Mark, Fodde, Riccardo
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755451/
https://ncbi.nlm.nih.gov/pubmed/29286377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56329
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