Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

We present a method to investigate the subcellular protein localization in the larval zebrafish retina by combining super-resolution light microscopy and scanning electron microscopy. The sub-diffraction limit resolution capabilities of super-resolution light microscopes allow improving the accuracy...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Mateos, José M., Barmettler, Gery, Doehner, Jana, Ojeda Naharros, Irene, Guhl, Bruno, Neuhauss, Stephan C.F., Kaech, Andres, Bachmann-Gagescu, Ruxandra, Ziegler, Urs
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Developmental Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755354/
https://ncbi.nlm.nih.gov/pubmed/29155784
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56113
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