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Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

We describe an efficient and reproducible protocol for the preparation of chromatin from adult mouse skeletal muscle, a physically resistant tissue with a high content of structural proteins. Dissected limb muscles from adult mice are physically disrupted by mechanical homogenisation, or a combinati...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Joshi, Shilpy, Ueberschlag-Pitiot, Vanessa, Metzger, Daniel, Davidson, Irwin
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755329/
https://ncbi.nlm.nih.gov/pubmed/29155791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56504
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