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A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation

Chromatin immunoprecipitation (ChIP) is a powerful method to determine protein binding to chromatin DNA. Fiber-rich skeletal muscle, however, has been a challenge for ChIP due to technical difficulty in isolation of high-quality nuclei with minimal contamination of myofibrils. Previous protocols hav...

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Bibliografische gegevens
Gepubliceerd in:J Vis Exp
Hoofdauteurs: Nohara, Kazunari, Chen, Zheng, Yoo, Seung-Hee
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MyJove Corporation 2017
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5609310/
https://ncbi.nlm.nih.gov/pubmed/28715394
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56013
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