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Isolation of Nuclei from Skeletal Muscle Satellite Cells and Myofibers for Use in Chromatin lmmunoprecipitation Assays

Studies investigating mechanisms controlling gene regulation frequently examine specific DNA sequences using chromatin immunoprecipitation (ChIP) assays to determine whether specific regulatory factors or modified histones are present. While use of primary cells or cell line models for differentiati...

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Bibliographische Detailangaben
Hauptverfasser: Ohkawa, Yasuyuki, Mallappa, Chandrashekara, Dacwag Vallaster, Caroline S., lmbalzano, Anthony N.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4201384/
https://ncbi.nlm.nih.gov/pubmed/22130858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-343-1_31
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