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Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells
Multinucleated myofibers are the functional contractile units of skeletal muscle. In adult muscle, mononuclear satellite cells, located between the basal lamina and the plasmalemma of the myofiber, are the primary myogenic stem cells. This chapter describes protocols for isolation, culturing and imm...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3633469/ https://ncbi.nlm.nih.gov/pubmed/23179849 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-128-8_28 |
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