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Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells

Multinucleated myofibers are the functional contractile units of skeletal muscle. In adult muscle, mononuclear satellite cells, located between the basal lamina and the plasmalemma of the myofiber, are the primary myogenic stem cells. This chapter describes protocols for isolation, culturing and imm...

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Autors principals: Keire, Paul, Shearer, Andrew, Shefer, Gabi, Yablonka-Reuveni, Zipora
Format: Artigo
Idioma:Inglês
Publicat: 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3633469/
https://ncbi.nlm.nih.gov/pubmed/23179849
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-128-8_28
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