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Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells

Multinucleated myofibers are the functional contractile units of skeletal muscle. In adult muscle, mononuclear satellite cells, located between the basal lamina and the plasmalemma of the myofiber, are the primary myogenic stem cells. This chapter describes protocols for isolation, culturing and imm...

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Bibliografiske detaljer
Main Authors: Keire, Paul, Shearer, Andrew, Shefer, Gabi, Yablonka-Reuveni, Zipora
Format: Artigo
Sprog:Inglês
Udgivet: 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3633469/
https://ncbi.nlm.nih.gov/pubmed/23179849
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-128-8_28
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