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Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle
We describe an efficient and reproducible protocol for the preparation of chromatin from adult mouse skeletal muscle, a physically resistant tissue with a high content of structural proteins. Dissected limb muscles from adult mice are physically disrupted by mechanical homogenisation, or a combinati...
Uloženo v:
| Vydáno v: | J Vis Exp |
|---|---|
| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
MyJove Corporation
2017
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5755329/ https://ncbi.nlm.nih.gov/pubmed/29155791 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56504 |
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