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Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

The goal of this protocol is to study mitochondria within intraepidermal nerve fibers. Therefore, 3D imaging and analysis techniques were developed to isolate nerve-specific mitochondria and evaluate disease-induced alterations of mitochondria in the distal tip of sensory nerves. The protocol combin...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Hamid, Hussein S., Hayes, John M., Feldman, Eva L., Lentz, Stephen I.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5752341/
https://ncbi.nlm.nih.gov/pubmed/28994751
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53369
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