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Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

The goal of this protocol is to study mitochondria within intraepidermal nerve fibers. Therefore, 3D imaging and analysis techniques were developed to isolate nerve-specific mitochondria and evaluate disease-induced alterations of mitochondria in the distal tip of sensory nerves. The protocol combin...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Main Authors: Hamid, Hussein S., Hayes, John M., Feldman, Eva L., Lentz, Stephen I.
Formato: Artigo
Idioma:Inglês
Publicado: MyJove Corporation 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5752341/
https://ncbi.nlm.nih.gov/pubmed/28994751
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53369
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