Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

For several decades, 5-methylcytosine (5mC) has been thought to be the only DNA modification with a functional significance in metazoans. The discovery of enzymatic oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) as well as detection of N6-met...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Ramsawhook, Ashley H., Lewis, Lara C., Eleftheriou, Maria, Abakir, Abdulkadir, Durczak, Paulina, Markus, Robert, Rajani, Seema, Hannan, Nicholas R.F., Coyle, Beth, Ruzov, Alexey
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Genetics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5752195/
https://ncbi.nlm.nih.gov/pubmed/28930980
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56318
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