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ITC Analysis of Ligand Binding to PreQ(1) Riboswitches
Riboswitches regulate genes by binding to small-molecule effectors. Isothermal titration calorimetry (ITC) provides a label-free method to quantify the equilibrium association constant, K(A), of a riboswitch interaction with its cognate ligand. In addition to probing affinity and specific chemical c...
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| Publicado en: | Methods Enzymol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2014
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5749633/ https://ncbi.nlm.nih.gov/pubmed/25432759 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-801122-5.00018-0 |
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