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Quantification of M13 and T7 bacteriophages by TaqMan and SYBR Green qPCR

TaqMan and SYBR Green quantitative PCR (qPCR) methods were developed as DNA-based approaches to reproducibly enumerate M13 and T7 phages from phage display selection experiments individually and simultaneously. The genome copies of M13 and T7 phages were quantified by TaqMan or SYBR Green qPCR refer...

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Detalles Bibliográficos
Publicado en:J Virol Methods
Autores principales: Peng, Xiujuan, Nguyen, Alex, Ghosh, Debadyuti
Formato: Artigo
Lenguaje:Inglês
Publicado: 2017
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5745055/
https://ncbi.nlm.nih.gov/pubmed/29196210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2017.11.012
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