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MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay

In the present work, we optimised and evaluated a qPCR system integrating 6-FAM (6-carboxyfluorescein)-labelled TaqMan probes and melting analysis using the SYTO 82 (S82) DNA binding dye in a single reaction. We investigated the influence of the S82 on various TaqMan and melting analysis parameters...

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Dettagli Bibliografici
Pubblicato in:PLoS One
Autori principali: Nagy, Alexander, Černíková, Lenka, Vitásková, Eliška, Křivda, Vlastimil, Dán, Ádám, Dirbáková, Zuzana, Jiřincová, Helena, Procházka, Bohumír, Sedlák, Kamil, Havlíčková, Martina
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4816343/
https://ncbi.nlm.nih.gov/pubmed/27031831
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0151204
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