MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay

In the present work, we optimised and evaluated a qPCR system integrating 6-FAM (6-carboxyfluorescein)-labelled TaqMan probes and melting analysis using the SYTO 82 (S82) DNA binding dye in a single reaction. We investigated the influence of the S82 on various TaqMan and melting analysis parameters...

Ful tanımlama

Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:PLoS One
Asıl Yazarlar: Nagy, Alexander, Černíková, Lenka, Vitásková, Eliška, Křivda, Vlastimil, Dán, Ádám, Dirbáková, Zuzana, Jiřincová, Helena, Procházka, Bohumír, Sedlák, Kamil, Havlíčková, Martina
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Public Library of Science 2016
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4816343/
https://ncbi.nlm.nih.gov/pubmed/27031831
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0151204
Etiketler: Etiketle
Etiket eklenmemiş, İlk siz ekleyin!

Benzer Materyaller