Tissue- and time-directed electroporation of CAS9 protein–gRNA complexes in vivo yields efficient multigene knockout for studying gene function in regeneration

A rapid method for temporally and spatially controlled CRISPR-mediated gene knockout in vertebrates will be an important tool to screen for genes involved in complex biological phenomena like regeneration. Here we show that in vivo injection of CAS9 protein–guide RNA (gRNA) complexes into the spinal...

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Vydáno v:NPJ Regen Med
Hlavní autoři: Fei, Ji-Feng, Knapp, Dunja, Schuez, Maritta, Murawala, Prayag, Zou, Yan, Pal Singh, Sumeet, Drechsel, David, Tanaka, Elly M
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2016
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5744710/
https://ncbi.nlm.nih.gov/pubmed/29302334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/npjregenmed.2016.2
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