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CRISPR–Cas9-mediated functional dissection of 3′-UTRs

Many studies using reporter assays have demonstrated that 3′ untranslated regions (3′-UTRs) regulate gene expression by controlling mRNA stability and translation. Due to intrinsic limitations of heterologous reporter assays, we sought to develop a gene editing approach to investigate the regulatory...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Zhao, Wenxue, Siegel, David, Biton, Anne, Tonqueze, Olivier Le, Zaitlen, Noah, Ahituv, Nadav, Erle, David J.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5737544/
https://ncbi.nlm.nih.gov/pubmed/28985357
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx675
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