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An LC-MS Chemical Derivatization Method for the Measurement of Five Different One-carbon States of Cellular Tetrahydrofolate

The cofactor tetrahydrofolate (THF) is used to reduce, oxidize and transfer one-carbon (1C) units required for the synthesis of nucleotides, glycine, and methionine. Measurement of intracellular THF species is complicated by their chemical instability, signal dilution caused by variable polyglutamat...

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Detalhes bibliográficos
Publicado no:Anal Bioanal Chem
Main Authors: Chen, Li, Ducker, Gregory S., Lu, Wenyun, Teng, Xin, Rabinowitz, Joshua D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5737010/
https://ncbi.nlm.nih.gov/pubmed/28799108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-017-0514-4
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