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Site-specific chromosomal gene insertion: Flp recombinase versus Cas9 nuclease

Site-specific recombination systems like those based on the Flp recombinase proved themselves as efficient tools for cell line engineering. The recent emergence of designer nucleases, especially RNA guided endonucleases like Cas9, has considerably broadened the available toolbox for applications lik...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Phan, Quang Vinh, Contzen, Jörg, Seemann, Petra, Gossen, Manfred
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5736728/
https://ncbi.nlm.nih.gov/pubmed/29259215
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-17651-0
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