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Site-specific chromosomal gene insertion: Flp recombinase versus Cas9 nuclease

Site-specific recombination systems like those based on the Flp recombinase proved themselves as efficient tools for cell line engineering. The recent emergence of designer nucleases, especially RNA guided endonucleases like Cas9, has considerably broadened the available toolbox for applications lik...

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Publicat a:Sci Rep
Autors principals: Phan, Quang Vinh, Contzen, Jörg, Seemann, Petra, Gossen, Manfred
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2017
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5736728/
https://ncbi.nlm.nih.gov/pubmed/29259215
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-17651-0
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