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Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv

CyDisCo is a system facilitating disulfide bond formation in recombinant proteins in the cytoplasm of Escherichia coli. Previously we screened for soluble expression of single chain antibody fragments (scFv) in the cytoplasm of E. coli in the presence and absence of CyDisCo, with >90% being solub...

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Veröffentlicht in:PLoS One
Hauptverfasser: Gąciarz, Anna, Ruddock, Lloyd W.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5734687/
https://ncbi.nlm.nih.gov/pubmed/29253024
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0189964
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