Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Single-molecule localization microscopy (SMLM) can visualize biological targets on the nanoscale, but complex hardware is required to perform SMLM in thick samples. Here, we combine 3D DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) with spinning disk confocal (SDC) hardware...

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Detalles Bibliográficos
Publicado en:Nat Commun
Main Authors: Schueder, Florian, Lara-Gutiérrez, Juanita, Beliveau, Brian J., Saka, Sinem K., Sasaki, Hiroshi M., Woehrstein, Johannes B., Strauss, Maximilian T., Grabmayr, Heinrich, Yin, Peng, Jungmann, Ralf
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2017
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5727263/
https://ncbi.nlm.nih.gov/pubmed/29233999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-02028-8
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