Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Single-molecule localization microscopy (SMLM) can visualize biological targets on the nanoscale, but complex hardware is required to perform SMLM in thick samples. Here, we combine 3D DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) with spinning disk confocal (SDC) hardware...

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Udgivet i:Nat Commun
Main Authors: Schueder, Florian, Lara-Gutiérrez, Juanita, Beliveau, Brian J., Saka, Sinem K., Sasaki, Hiroshi M., Woehrstein, Johannes B., Strauss, Maximilian T., Grabmayr, Heinrich, Yin, Peng, Jungmann, Ralf
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5727263/
https://ncbi.nlm.nih.gov/pubmed/29233999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-02028-8
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