Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Single-molecule localization microscopy (SMLM) can visualize biological targets on the nanoscale, but complex hardware is required to perform SMLM in thick samples. Here, we combine 3D DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) with spinning disk confocal (SDC) hardware...

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Détails bibliographiques
Publié dans:Nat Commun
Auteurs principaux: Schueder, Florian, Lara-Gutiérrez, Juanita, Beliveau, Brian J., Saka, Sinem K., Sasaki, Hiroshi M., Woehrstein, Johannes B., Strauss, Maximilian T., Grabmayr, Heinrich, Yin, Peng, Jungmann, Ralf
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2017
Sujets:
Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5727263/
https://ncbi.nlm.nih.gov/pubmed/29233999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-02028-8
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