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Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer

Here, we employed cDNA amplicon sequencing using a long-read portable sequencer, MinION, to characterize various types of mutations in cancer-related genes, namely, EGFR, KRAS, NRAS and NF1. For homozygous SNVs, the precision and recall rates were 87.5% and 91.3%, respectively. For previously report...

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Publicat a:DNA Res
Autors principals: Suzuki, Ayako, Suzuki, Mizuto, Mizushima-Sugano, Junko, Frith, Martin C, Makałowski, Wojciech, Kohno, Takashi, Sugano, Sumio, Tsuchihara, Katsuya, Suzuki, Yutaka
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5726485/
https://ncbi.nlm.nih.gov/pubmed/29117310
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/dnares/dsx027
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