GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

BACKGROUND: State-of-the-art strain engineering techniques for the host Pichia pastoris (syn. Komagataella spp.) include overexpression of homologous and heterologous genes, and deletion of host genes. For metabolic and cell engineering purposes the simultaneous overexpression of more than one gene...

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Vydáno v:BMC Syst Biol
Hlavní autoři: Prielhofer, Roland, Barrero, Juan J., Steuer, Stefanie, Gassler, Thomas, Zahrl, Richard, Baumann, Kristin, Sauer, Michael, Mattanovich, Diethard, Gasser, Brigitte, Marx, Hans
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2017
Témata:
Methodology Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5723102/
https://ncbi.nlm.nih.gov/pubmed/29221460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12918-017-0492-3
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