The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography

The crystal structure of the trans-acyltrans-ferase (AT) from the disorazole polyketide synthase (PKS) was determined at room temperature to a resolution of 2.5 Å using a new method for the direct delivery of the sample into an X-ray free-electron laser. A novel sample extractor efficiently delivere...

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Udgivet i:Biochemistry
Main Authors: Mathews, Irimpan I., Allison, Kim, Robbins, Thomas, Lyubimov, Artem Y., Uervirojnangkoorn, Monarin, Brunger, Axel T., Khosla, Chaitan, DeMirci, Hasan, McPhillips, Scott E., Hollenbeck, Michael, Soltis, Michael, Cohen, Aina E.
Format: Artigo
Sprog:Inglês
Udgivet: 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5721673/
https://ncbi.nlm.nih.gov/pubmed/28832129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.7b00711
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