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Nm-seq maps 2′-O-methylation sites in human mRNA with base precision
The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate o...
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| Publicado no: | Nat Methods |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5712428/ https://ncbi.nlm.nih.gov/pubmed/28504680 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4294 |
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