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Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate o...

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Detaylı Bibliyografya
Yayımlandı:Nat Methods
Asıl Yazarlar: Dai, Qing, Moshitch-Moshkovitz, Sharon, Han, Dali, Kol, Nitzan, Amariglio, Ninette, Rechavi, Gideon, Dominissini, Dan, He, Chuan
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2017
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5712428/
https://ncbi.nlm.nih.gov/pubmed/28504680
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4294
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