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Accurate, Streamlined Analysis of mRNA Translation by Sucrose Gradient Fractionation

The efficiency with which proteins are produced from mRNA molecules can vary widely across transcripts, cell types, and cellular states. Methods that accurately assay the translational efficiency of mRNAs are critical to gaining a mechanistic understanding of post-transcriptional gene regulation. On...

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Bibliographische Detailangaben
Veröffentlicht in:Bio Protoc
Hauptverfasser: Aboulhouda, Soufiane, Di Santo, Rachael, Therizols, Gabriel, Weinberg, David
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5697790/
https://ncbi.nlm.nih.gov/pubmed/29170751
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2573
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