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Quantitative analysis of mRNA translation in mammalian spermatogenic cells with sucrose and Nycodenz gradients

BACKGROUND: Developmental and global regulation of mRNA translation plays a major role in regulating gene expression in mammalian spermatogenic cells. Sucrose gradients are widely used to analyze mRNA translation. Unfortunately, the information from sucrose gradient experiments is often compromised...

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Main Authors: Kleene, Kenneth C, Bagarova, Jana, Hawthorne, Sabrina K, Catado, Leah M
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3022843/
https://ncbi.nlm.nih.gov/pubmed/21184686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-7827-8-155
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