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Streamlined Protocol for mRNA Display
mRNA display is a powerful method for in vitro directed evolution of polypeptides, but its time-consuming, technically demanding nature has hindered its widespread use. We present a streamlined protocol in which lengthy mRNA purification steps are replaced with faster precipitation and ultrafiltrati...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3666848/ https://ncbi.nlm.nih.gov/pubmed/23305392 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/co300135r |
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