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Streamlined Protocol for mRNA Display

mRNA display is a powerful method for in vitro directed evolution of polypeptides, but its time-consuming, technically demanding nature has hindered its widespread use. We present a streamlined protocol in which lengthy mRNA purification steps are replaced with faster precipitation and ultrafiltrati...

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Bibliografski detalji
Glavni autori: Barendt, Pamela A., Ng, Daphne T.W., McQuade, Casey N., Sarkar, Casim A.
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3666848/
https://ncbi.nlm.nih.gov/pubmed/23305392
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/co300135r
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