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Streamlined Protocol for mRNA Display
mRNA display is a powerful method for in vitro directed evolution of polypeptides, but its time-consuming, technically demanding nature has hindered its widespread use. We present a streamlined protocol in which lengthy mRNA purification steps are replaced with faster precipitation and ultrafiltrati...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2013
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3666848/ https://ncbi.nlm.nih.gov/pubmed/23305392 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/co300135r |
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