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Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the intracellular protein abundance by Western blot along with the measure...
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| Publicado no: | Bio Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5697766/ https://ncbi.nlm.nih.gov/pubmed/29170750 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2396 |
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