Revealing dynamics of helicase translocation on single-stranded DNA using high-resolution nanopore tweezers

Enzymes that operate on DNA or RNA perform the core functions of replication and expression in all of biology. To gain high-resolution access to the detailed mechanistic behavior of these enzymes, we developed single-molecule picometer-resolution nanopore tweezers (SPRNT), a single-molecule techniqu...

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Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Craig, Jonathan M., Laszlo, Andrew H., Brinkerhoff, Henry, Derrington, Ian M., Noakes, Matthew T., Nova, Ian C., Tickman, Benjamin I., Doering, Kenji, de Leeuw, Noah F., Gundlach, Jens H.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2017
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5692567/
https://ncbi.nlm.nih.gov/pubmed/29078357
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1711282114
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