Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal

We present protein-PAINT – the implementation of the general principles of PAINT (Point Accumulation for Imaging in Nanoscale Topography) for live-cell protein labeling. Our method employs the specific binding of cell-permeable fluorogenic dyes to genetically encoded protein tags. We engineered thre...

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Dettagli Bibliografici
Pubblicato in:Chem Sci
Autori principali: Bozhanova, Nina G., Baranov, Mikhail S., Klementieva, Natalia V., Sarkisyan, Karen S., Gavrikov, Alexey S., Yampolsky, Ilia V., Zagaynova, Elena V., Lukyanov, Sergey A., Lukyanov, Konstantin A., Mishin, Alexander S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Royal Society of Chemistry 2017
Soggetti:
Chemistry
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5676496/
https://ncbi.nlm.nih.gov/pubmed/29147545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7sc01628j
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