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Tryptophan-based chromophore in fluorescent proteins can be anionic

Cyan fluorescent proteins (CFP) with tryptophan66-based chromophore are widely used for live cell imaging. In contrast to green and red fluorescent proteins, no charged states of the CFP chromophore have been described. Here, we studied synthetic CFP chromophore and found that its indole group can b...

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Bibliographic Details
Main Authors: Sarkisyan, Karen S., Yampolsky, Ilia V., Solntsev, Kyril M., Lukyanov, Sergey A., Lukyanov, Konstantin A., Mishin, Alexander S.
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 2012
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3429880/
https://ncbi.nlm.nih.gov/pubmed/22934131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep00608
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