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Tryptophan-based chromophore in fluorescent proteins can be anionic

Cyan fluorescent proteins (CFP) with tryptophan66-based chromophore are widely used for live cell imaging. In contrast to green and red fluorescent proteins, no charged states of the CFP chromophore have been described. Here, we studied synthetic CFP chromophore and found that its indole group can b...

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Main Authors: Sarkisyan, Karen S., Yampolsky, Ilia V., Solntsev, Kyril M., Lukyanov, Sergey A., Lukyanov, Konstantin A., Mishin, Alexander S.
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group 2012
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3429880/
https://ncbi.nlm.nih.gov/pubmed/22934131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep00608
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