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Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal
We present protein-PAINT – the implementation of the general principles of PAINT (Point Accumulation for Imaging in Nanoscale Topography) for live-cell protein labeling. Our method employs the specific binding of cell-permeable fluorogenic dyes to genetically encoded protein tags. We engineered thre...
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| Publicado no: | Chem Sci |
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| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Royal Society of Chemistry
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5676496/ https://ncbi.nlm.nih.gov/pubmed/29147545 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7sc01628j |
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