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Real-time Analysis of Folding Upon Binding of a Disordered Protein using Dissolution DNP NMR

The kinase inhibitory domain of the cell cycle regulatory protein p27(Kip1) (p27) was nuclear spin hyperpolarized using dissolution dynamic nuclear polarization (D-DNP). While intrinsically disordered in isolation, p27 adopts secondary structure, including α-helical structure, upon binding to cyclin...

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Bibliografski detalji
Izdano u:Angew Chem Int Ed Engl
Glavni autori: Ragavan, Mukundan, Iconaru, Luigi I., Park, Cheon-Gil, Kriwacki, Richard W., Hilty, Christian
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5674774/
https://ncbi.nlm.nih.gov/pubmed/28508552
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201700464
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