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Real-time Analysis of Folding Upon Binding of a Disordered Protein using Dissolution DNP NMR
The kinase inhibitory domain of the cell cycle regulatory protein p27(Kip1) (p27) was nuclear spin hyperpolarized using dissolution dynamic nuclear polarization (D-DNP). While intrinsically disordered in isolation, p27 adopts secondary structure, including α-helical structure, upon binding to cyclin...
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| Publicado no: | Angew Chem Int Ed Engl |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5674774/ https://ncbi.nlm.nih.gov/pubmed/28508552 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201700464 |
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