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Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt-Ended DNA Breaks by In Situ Ligation

In situ ligation (ISL) is a simple and specific technique for apoptosis labeling in tissue sections. In its most economical version ISL uses ordinary PCR-labeled DNA fragments as probes. In tissue sections these makeshift probes are ligated to apoptotic DNA breaks by T4 DNA ligase. The approach can...

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Bibliografiske detaljer
Udgivet i:Methods Mol Biol
Hovedforfatter: Didenko, Vladimir V.
Format: Artigo
Sprog:Inglês
Udgivet: 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5673075/
https://ncbi.nlm.nih.gov/pubmed/28710763
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7187-9_15
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