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IN SITU LIGATION SIMPLIFIED: USING PCR FRAGMENTS FOR DETECTION OF DOUBLE-STRAND DNA BREAKS IN TISSUE SECTIONS
The simplified in situ ligation procedure is described. All reagents for the assay can be easily obtained in any molecular or cell biology laboratory. The technique uses ligation of double-stranded, PCR-derived DNA fragments labeled with digoxigenin or fluorophores for highly selective detection of...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3324586/ https://ncbi.nlm.nih.gov/pubmed/21057921 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-409-8_6 |
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